Optimization of Lateral Flow Assay for the Dual-detection of Mycotoxins

Moon, Jihea; Kim, Giyoung; Lee, Sangdae; Park, Saetbyeol; Kim, Hyeokju

doi:10.13050/foodengprog.2012.16.3.214

Food Eng. Prog. 2012; 16(3):214-218

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2012.16.3.214

Article

곰팡이 독소 동시 분석용 간이진단키트 최적화 연구

문지혜¹, 김기영¹^,^*, 이상대¹, 박샛별¹, 김혁주¹

Optimization of Lateral Flow Assay for the Dual-detection of Mycotoxins

Jihea Moon¹, Giyoung Kim¹^,^*, Sangdae Lee¹, Saetbyeol Park¹, Hyeokju Kim¹

Author Information & Copyright ▼

¹국립농업과학원 농업공학부

¹Department of Agricultural Engineering, National Academy of Agricultural Sciences

^*Corresponding author: Giyoung Kim, Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suin-ro, 126th, Gwonseon-gu, Suwon, 442-707, Korea. Tel: +82-31-290-1899; Fax: +82-31-299-1900, E-mail: giyoung@korea.kr

ⓒ Copyright 2012 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 10, 2012; Revised: Aug 21, 2012; Accepted: Aug 21, 2012

Published Online: Aug 31, 2012

Abatract

This study was performed to develop and optimize a rapid strip kit for the dual-detection of Aflatoxin B₁ (AF) and Ochratoxin A (OTA). The strip kit is composed of sample pad, conjugation pad, nitrocellulose membrane, and absorbent pad. Manually spotted mycotoxin-BSA conjugates and anti-mouse IgG on nitrocellulose membrane were used as the test line and the control line, respectively. Conditions for rapid and easy detection of mycotoxins, conjugation between toxin-MAbs and nano Au particles, pretreatment method of pads, and composition of standards solutions were optimized. Feasibility of the strip kit to detect AF and OTA were evaluated with standard samples. Test results were acquired in 10 min and the detection limits were 100 ppm for AF and OTA, respectively.

Keywords: aflatoxin B₁; ochratoxin A; lateral flow assay; mycotoxins; biosensor

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곰팡이 독소 동시 분석용 간이진단키트 최적화 연구

Abatract