Purification of Carp Serum Metallothionein Induced in Carp with Cadmium and Production of Polyclonal Antibody

Ryu, Hyung-Seok; Kang, Ho-Joon; Kim, Seul Ki; Kim, Namsoo; , 김우연

doi:10.13050/foodengprog.2009.13.4.314

Food Eng. Prog. 2009; 13(4):314-319

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2009.13.4.314

Article

카드뮴 유도 잉어 혈액으로부터의 Metallothionein 정제 및 이에 대한 다클론 항체 생산

유형석¹, 강호준¹, 김슬기¹, 김남수², 김우연¹^,^*

Purification of Carp Serum Metallothionein Induced in Carp with Cadmium and Production of Polyclonal Antibody

Hyung-Seok Ryu¹, Ho-Joon Kang¹, Seul Ki Kim¹, Namsoo Kim², Woo-Yeon Kim ¹^,^*

Author Information & Copyright ▼

¹중앙대학교 생명공학과

²한국식품연구원

¹Department of Biotechnology, Chung-Ang University, Anseong 456-756, Korea

²Korea Food Research Institute, Seongnam 463-746, Korea

^*Corresponding author: Woo-Yeon Kim, Department of Biotechnology College of Industrial Sciences, Chung-Ang University Anseong, Gyunggido, 456-756, Republic of Korea, Tel: +82-31-675-3063; Fax: +82-31-675-0405, E-mail: wykim@cau.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 13, 2009; Revised: Nov 16, 2009; Accepted: Nov 16, 2009

Published Online: Nov 30, 2009

Abatract

Metallothioneins (MTs) are low molecular weight, cysteine-rich, and heavy metal binding proteins, which could be induced with heavy metals such as Cd, Hg, Zn and Cu in liver, kidney, and in cultured cells. By using ion exchange chromatography on DE-52, MT was purified from the serum of carp induced with cadmium in order to produce antibody against MT. Polyclonal antibody produced against purified carp MT reacted well with MT in the serum of carp induced with cadmium, whereas control serum did not. This may indicate that the polyclonal antibody against the carp MT could be used for the preparation of biosensors to detect MT in fishes like carp.

Keywords: carp metallothionein; purification; antibody; reactivity

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카드뮴 유도 잉어 혈액으로부터의 Metallothionein 정제 및 이에 대한 다클론 항체 생산

Abatract