Development of Rapid Detection Kit for Aflatoxin B1 in Cereals

Choi, Sunmi; Kim, Giyoung; Lee, Sangdae; Moon, Jihea; Om, Aeson

doi:10.13050/foodengprog.2013.17.3.271

Food Eng. Prog. 2013; 17(3):271-275

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2013.17.3.271

Article

곡류 중 아플라톡신 B1 신속 검출용 간이진단키트의 개발

최선미², 김기영¹, 이상대¹, 문지혜¹, 엄애선²^,^*

Development of Rapid Detection Kit for Aflatoxin B1 in Cereals

Sunmi Choi², Giyoung Kim¹, Sangdae Lee¹, Jihea Moon¹, Aeson Om²^,^*

Author Information & Copyright ▼

¹농촌진흥청 국립농업과학원 농업공학부

²한양대학교 식품영양학과

¹Department of Agricultural Engineering, National Academy of Agricultural Science, Rural Development Administration

²Department of Food and Nutrition, Hanyang University

^*Corresponding author: Aeson Om, Department of Food and Nutrition, College of Human Ecology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 133-791, Korea, Tel: +82-2-2220-1203; Fax: +82-2-2220-1856, E-mail: aesonom@hanyang.ac.kr

ⓒ Copyright 2013 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 14, 2013; Revised: Aug 20, 2013; Accepted: Aug 23, 2013

Published Online: Aug 31, 2013

Abatract

The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 μg/kg) for Aflatoxin B₁ (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 μg/kg for both. This result is lower than the LOD of KFDA in food (10 μg/kg) and satisfies the LOD of EU (2-12 μg/kg) and Japan (10 μg/kg) in food.

Keywords: Aflatoxin B1; Rapid detection kit; Cereals; Corn

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곡류 중 아플라톡신 B1 신속 검출용 간이진단키트의 개발

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