Article

한외여과법에 의한 미강단백질 농축물의 분리에 관한 연구

이의석1, 홍순택1,*
Eui-Seok Lee1, Soon-Taek Hong1,*
Author Information & Copyright
1충남대학교 농업생명과학대학 식품공학과 식품에멀젼 실험실
1Laboratory of food emulsion, Department of Food Science and Technology, College of Agriculture and Life science, Chungnam National University
*Corresponding author: Soon-Taek Hong, Department of Food Science and Technology, College of Agriculture and Life science, Chungnam National University, 99 Daehak-ro(St), Yuseong-gu, Daejeon 305-764, Korea. Tel: +82-42-821-6727; Fax: +82-42-821-8900, E-mail: Hongst@cnu.ac.kr

ⓒ Copyright 2013 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 26, 2013; Revised: May 24, 2013; Accepted: May 24, 2013

Published Online: May 31, 2013

Abatract

In this study, ultrafiltration with a membrane of molecular weight cut off (MWCO) 50 kDa or 100 kDa was applied to produce rice bran protein concentrate and influence process variables such as operation pressure, time, and volume concentration ratio (VCR) on the composition of rice bran protein concentrate and changes in permeation flux were investigated. An increase in permeation flux was observed in accordance with increasing operation pressure, while it decreased with operation time, possibly due to concentration polarization. In addition, the permeation flux was observed to be higher when a membrane with larger MWCO was used and a maximum permeation flux (32 L/m2·h) was observed with an operation pressure of 2 kgf/cm2 and a 100 kDa membrane. Protein content in the concentrate rose with increasing VCR, and also with membranes of smaller MWCO (i.e, 50 kDa). It was found at VCR 8 and with an operation pressure of 2 kgf/cm2 that total processing time decreased by 40% and average permeation flux increased by about 2 times, when a membrane with 2 times larger MWCO (i.e., 50 kDa→100 kDa) was used.

Keywords: ultrafiltration; Rice bran protein concentrate; molecular weight cut off