한외여과법에 의한 미강단백질 농축물의 분리에 관한 연구
Received: Apr 26, 2013; Revised: May 24, 2013; Accepted: May 24, 2013
Published Online: May 31, 2013
Abatract
In this study, ultrafiltration with a membrane of molecular weight cut off (MWCO) 50 kDa or 100 kDa was applied to produce rice bran protein concentrate and influence process variables such as operation pressure, time, and volume concentration ratio (VCR) on the composition of rice bran protein concentrate and changes in permeation flux were investigated. An increase in permeation flux was observed in accordance with increasing operation pressure, while it decreased with operation time, possibly due to concentration polarization. In addition, the permeation flux was observed to be higher when a membrane with larger MWCO was used and a maximum permeation flux (32 L/m2·h) was observed with an operation pressure of 2 kgf/cm2 and a 100 kDa membrane. Protein content in the concentrate rose with increasing VCR, and also with membranes of smaller MWCO (i.e, 50 kDa). It was found at VCR 8 and with an operation pressure of 2 kgf/cm2 that total processing time decreased by 40% and average permeation flux increased by about 2 times, when a membrane with 2 times larger MWCO (i.e., 50 kDa→100 kDa) was used.