Article

팽화 홍삼으로부터 20(S)-Ginsenoside Rg3와 Rg5의 분리 및 구조동정

안영은1, 조진경2, 백남인2, 최성원3, 허남윤3, 박석준4, 김병용1, 백무열1,*
Young-Eun An1, Jin-Gyeong Cho2, Nam-In Baik2, Sung-Won Choi3, Nam-Yoon Hur3, Seok-Jun Park4, Byung-Yong Kim1, Moo-Yeol Baik1,*
Author Information & Copyright
1경희대학교 생명자원과학연구원 식품공학과
3경희대학교 한방재료가공학과
3오산대학교 호텔조리계열
4CJ(주) 식품연구소
1Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University
2Graduate School of Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University
3Hotel and Culinary Arts Division, Osan University
4CJ Foods R&D
*Corresponding author: Moo-Yeol Baik, Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Gyeonggi-do 449-701, Korea, Tel: +82-31-201-2625; Fax: +82-31-204-8116, E-mail: mooyeol@khu.ac.kr

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 30, 2010; Revised: May 20, 2010; Accepted: May 24, 2010

Published Online: May 31, 2010

Abatract

Red ginseng tail roots (9.8 g water/100 g sample) were puffed at 7, 8, 9, and 10 kgf/cm2 using a rotational puffing gun. Puffed red ginseng was extracted with 70% ethanol, and the concentrated extract was successively partitioned with diethyl ether, n-butanol and H2O. Two unknown ginsenosides from puffed red ginseng were found at 63 and 65 min of retention time in HPLC chromatogram suggesting that chemical structure of some ginsenosides might be altered during the puffing process. Identification of two unknown compounds was carried out using TLC, HPLC and NMR. Two major compounds were isolated from TLC. According to TLC result, compound I was expected to be the mixture of ginsenosides Rk1 and Rg5, and compound II was expected to be a 20(S)-ginsenoside Rg3. Three compounds were isolated from n-butanol fraction through repeated silica gel and octadecyl silica gel column chromatographies. From the result of 1H- and 13C-NMR data, the chemical structures of unknown compounds were determined as ginsenoside Rg5 and 20(S)-ginsenoside Rg3. Unfortunately, ginsenoside Rk1 could not be separated from ginsenoside-Rg5 in the compound I. It was carefully reexamined using HPLC and confirmed that the last unknown compound was ginsenoside-Rk1.

Keywords: red ginseng; puffing; ginsenoside; high performance liguid chromatography; thin layer chromatography; nuclear magnetic resonance