CO₂를 이용한 초임계 유체 추출과 초고압 전처리 공정의 복합공정을 사용한 대두 분말의 대두유 추출 수율 최적화

Response surface methodology (RSM) was applied to determine the optimum operating conditions to maximize the yield of oil components from the soy bean powders using a novel combined process of a supercritical CO₂ fluid extraction (SCFE) with a high pressure process (HPP) as a pretreatment. The optimization of the combined process was conducted with two steps: 1) determining the optimum conditions for maximizing the yield under the SCFE, and 2) determining the optimum conditions for HPP at the optimum operating conditions of SCFE determined from the step 1. Central composite design was applied for both steps of optimization. The process variables for SCFE were ranged from 200-400 bar, 40-70°C, and 20-40 g/min) for the pressure of extraction vessel, temperature, and mass flow rate, respectively. In addition the process variables for HPP were the applied pressure (100-400 MPa) and the applied time (5-25 min). The maximum yield from SCFE was 25.88% at 468.18 bar, 80.23°C, and 46.82 g/min. The optimum operating conditions of HPP in the combined process was predicted to be 462.13 MPa and 1 min. for 24.91% of yield. The yield of the combined process was lower than that of SCFE. It might be because of the increase of particle size due to the agglomeration after applying HPP.