반응표면분석법(RSM)을 이용한 두릅의 유용성분 환류추출공정의 최적화

Response surface methodology (RSM) was used to monitor the characteristics of a return current extracts from Aralia elata. A central composite design was used to investigate the effects of the independent variables of extraction time (X₁), ethanol concentration (X₂) and extraction temperature (X₃) on the dependent variables yield (Y₁), total phenolics (Y₂), electron donating ability (Y₃), browning color (Y₄) and reducing sugar contents (Y₅) of extracts. The maximum yield was at 316.58 min (X₁), 15.68% (X₂) and 93.49°C (X₃) in saddle point. Total phenolics were highly affected by ethanol concentration. The maximum total phenolics were at 94.78°C (X₁), 22.9% (X₂), 323.05 min (X₃) in saddle point. The maximum electron donating ability was at 94.78°C (X₁), 29.82% (X₂), 323.05 min (X₃) and was highly affected by ethanol concentration. The maximum browning color was at 97.00°C (X₁), 38.35% (X₂), 321.82 min (X₃). The browning color was highly affected by ethanol concentration. The ruducing sugar content was at 97.00°C, 38.35%, When the extraction temperature, ethanol concentration, and extraction time were 87.62°C (X₁), 24.62% (X₂), 339.39 min (X₃). The optimal conditions of extraction conditions for response variables by superimposing of contour maps were 263.5 min (X₁), 14% (X₂) and 89.5°C (X₃).