Isolation and Characterization of Thermophilic Microorganism Producing Starch-hydrolyze Enzyme

Choi, Wonseok; Bai, Dong-Hoon

doi:10.13050/foodengprog.2010.14.1.7

Food Eng. Prog. 2010; 14(1):7-13

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2010.14.1.7

Article

한국 토양으로부터 전분가수분해효소를 생산하는 고온성 균주의 선별과 동정

최원석¹, 배동훈¹^,^*

Isolation and Characterization of Thermophilic Microorganism Producing Starch-hydrolyze Enzyme

Wonseok Choi¹, Dong-Hoon Bai¹^,^*

Author Information & Copyright ▼

¹단국대학교 식품공학과

¹Department of Food Engineering, Dankook University

^*Corresponding author: Dong-Hoon Bai, Department of Food Engineering, Dankook University, Cheonan 330-714, Korea, Tel:+82-41-550-3562; Fax: +82-41-550-3566, E-mail: baidh@dankook.ac.kr

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 22, 2009; Revised: Nov 24, 2009; Accepted: Jan 12, 2010

Published Online: Feb 28, 2010

Abatract

A thermophilic microorganism, which is able to hydrolyze starch, was isolated from soil and compost in Korea. It was Gram-positive, rod-shaped, catalase positive, nonmotile, glucose and mannitol fermentative, xylose oxidative, and spore forming microorganism. It also has an ability to hydrolyze casein and gelatin. The color of colony was yellowish white. The sequence of 16S rDNA of strain 2719 showed 99.5% sequence homology with the sequence of 16S rDNA of Bacillus thermoglucosidasius. On the basis of biochemical and physiological properties and phylogenetic analysis, the isolated strain was named as Bacillus thermoglucosidasius 2719.

Keywords: Bacillus thermoglucosidasius; Thermophile; Amylase

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한국 토양으로부터 전분가수분해효소를 생산하는 고온성 균주의 선별과 동정

Abatract