감초추출물에 대한 Lactobacillus acidophilus KCCM 32820의 배양 특성

Aglycon in licorice converted from glycosides by fermentation has been implicated to improved physiological activity, this study was carried out to determine the aglycon production in the cultured licorice-extract broth by Lactobacillus acidophilus KCCM 32820. According to inoculum size, pH was decreased -0.32 after 6 days at 10⁶ CFU/ mL, pH and contents of the glycyrrhizin in culture broth were rapidly decreased after 2 days at 10⁷ CFU/mL and 10⁸ CFU/mL, the concentration of glycyrrhizin after 6 days were determined 27.5 mg/100 mL and 20.8 mg/100 mL, the utilization rates were 71.8%, 78.6%, respectively. Initial inoculum size should be above 10⁷ CFU/mL on the fermentation of licorice extracts, and immobilized L. acidophilus KCCM 32820 was faster decrease than free cell on the fermentation. The metabolites of fermented licorice extracts can be verified the improvement in efficacy of physiological functions, further research need to do.