고압에서 β-glucosidase 반응속도론 및 평형에 관한 연구

β-Glucosidase enzyme reaction under high hydrostatic pressure was investigated in terms of physical chemistry. A model substrate (p-nitrophenyl-β-D-glucopyranoside (pNPG)) was used, and the pressure effects on the enzymatic hydrolysis (pNPG→pNP) at 25 MPa, 50 MPa, 75 MPa, and 100 MPa were analyzed. Two parts of the reaction such as kinetic and equilibrium stages were considered for mathematical modelling, and their physicochemical parameters such as forward and inverse reaction constants, equilibrium constant, volume change by pressure, etc. were mathematically modeled. The product concentration increased with pressure, and the two stages of reaction were observed. Prediction models were derived to numerically compute the product concentrations according to reaction time over kinetic to equilibrium stages under high pressure condition. Conclusively, the β-glucosidase enzyme reaction could be activated by pressurization within 100 MPa, and the developed models were very successful in their prediction.