Salmonella 신속 검출용 간이진단키트의 성능 개선 연구

박샛별¹, 김기영¹^,^*, 문지혜¹

Improvement of Lateral Flow Assay for the rapid detection of Salmonella

Saetbyeol Park¹, Giyoung Kim¹^,^*, Jihea Moon¹

¹국립농업과학원 농업공학부

¹Department of Agricultural Engineering, National Academy of Agricultural Sciences

^*Corresponding author: Giyoung Kim, Department of Agricultural engineering, National Academy of Agricultural Sciences, Suin-ro, 126th, Gwonseon-gu, Suwon, 442-707, Korea. Tel: +82-31-290-1899; Fax: +82-31-299-1900, E-mail: giyoung@korea.kr

ⓒ Copyright 2012 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 28, 2012; Revised: Oct 12, 2012; Accepted: Oct 12, 2012

Published Online: Nov 30, 2012

Abatract

In this study, a rapid immuno-chromatographic assay was developed to detect Salmonella Typhimurium. This assay was based on the lateral flow immunoassay. Two different anti-Salmonella polyclonal antibody (Pab) are used in this strip assay for the double antibody sandwich immunoassay method. The strip sensor was composed of sample pad, conjugation pad, membrane, and absorbent pad. For the optimal conjugation between nano-gold and anti-Salmonella Pab, the pH value of colloidal gold solution was determined to 8.4. To prevent non-specific binding between nano gold and anti-Salmonella Pab, sample pad and membrane was treated with PBS buffer, included 2% BSA and 10% sucrose. The control and detection zones were visually detected according to antigen-antibody binding. The detection limit of the developed strip assay was 10⁵ CFU/mL within 10 min, and improved until 10⁴ CFU/mL after 12 hr.

Keywords: lateral flow immunoassay; strip sensor; salmonella