신선 과채류에 존재하는 박테리오신 유사 활성을 지니는 유산균의 분포와 다양성

박영서¹^,^*, 장재권², 최영진³, 정명수⁴, 박훈⁵, 심건섭⁶

Distribution and Biodiversity of Lactic Acid Bacteria Having Bacteriocin-like Activity from Fresh Fruits and Vegetables

Young-Seo Park¹^,^*, Jae Kweon Jang², Young Jin Choi³, Myong-Soo Chung⁴, Hoon Park⁵, Kun-Sub Shim⁶

¹경원대학교 식품생물공학과

²청강문화산업대학 식품과학과

³서울대학교 식품생명공학전공

⁴이화여자대학교 식품공학과

⁵선문대학교 식품과학과

⁶(주)그린바이오

¹Department of Food Science and Biotechnology, Kyungwon University

²Department of Food Science, Chungkang College of Cultural Industries

³Department of Agricultural Biotechnology, Seoul National University

⁴Department of Food Science and Engineering, Ewha Womans University

⁵Department of Food Science, Sunmoon University

⁶Greenbio Co., Ltd.

^*Corresponding author: Young-Seo Park, Department of Food Science and Biotechnology, Kyungwon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do 461-701, Korea, Tel: +82-31-750-5378; Fax: +82-31-750-5273, E-mail: ypark@kyungwon.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Dec 08, 2008; Revised: Jan 04, 2009; Accepted: Jan 15, 2009

Published Online: Feb 28, 2009

Abatract

From the 25 fresh fruits and vegetable products, 1,250 isolates grown on MRS agar media were screened for the inhibitory activity on the growth of Escherichia coli 0157:H7, Listeria monocytogenes, and Bacillus cereus as well as Lactobacillus plantarum, L. casei, and Lactococcus lactis subsp. lactis. Among them, 607 isolates (49% of total isolates) from 23 different foods produced growth inhibitory activity on the E. coli 0157:H7, L. monocytogenes, or B. cereus. When these isolates were screened for the inhibitory activity on the growth of L. plantarum, L. casei, and Lactococcus lactis subsp., 24 isolates (3% of total isolates) from 7 food samples showed bacteriocin-like activity. These isolates had typical physiological characteristics of lactic acid bacteria, which indicated these isolates were strains of lactic acid bacteria. The inhibitor from 3 out of 24 revealed as nicin. From the RAPD-PCR profiles, 24 strains was classified and it was also indicated that most of the strains isolated from same produce showed similar phylogenetic profile.

Keywords: lactic acid bacteria; bacteriocin-like activity; fruits and vegetables; biodiversity