Table 8 Effect of high-pressure enzyme treatment on removal starch, crude polysaccharide and water solubility depend on each condition

Treatment		Enzyme	Condition	2)RPSC (%)	SCPC (%)	WSI (%)	WAI (g/mL)	TS (%)	RS (%)
Time (h)	1)HP	Pec	1	3)22.38±0.21b	2.72±0.11b	61.26±0.47b	8.40±0.41a	33.68±0.29	17.12±0.60b
			2	23.03±0.51b	4.11±0.86a	70.67±0.42a	1.61±0.12c	35.33±2.99	21.86±0.08a
			3	24.64±0.46a	2.35±0.19b	53.29±1.25c	7.66±0.19b	33.86±1.74	16.34±0.09c
			F-value	23.33***	9.76*	347.32***	580.80***	N.S	216.50***
			Pec+Vis	1	21.00±0.40c	3.70±0.16	58.38±0.23a	7.90±0.15a	23.07±1.44b	16.75±0.54b
		2		23.99±0.16b	3.99±0.87	59.66±0.41a	2.16±0.07c	39.23±1.96a	25.80±0.55a
		3		24.78±0.51a	4.33±0.65	46.81±1.50b	7.45±0.19b	20.07±0.57c	16.77±0.51b
		F-value		81.00***	N.S	183.53***	1,431.86***	153.07***	285.52***
		WB		Pec	1	9.73±0.48c	4.81±0.11a	52.83±1.06	6.69±0.28b	16.53±0.46c	7.31±0.38c
			2		16.90±0.32b	4.92±1.17a	58.56±2.24a	1.86±0.10c	30.99±0.16a	21.23±0.01a
			3		21.78±0.33a	3.22±0.33b	41.51±0.43c	7.48±0.52a	21.28±1.36b	16.27±1.12b
	F-value		748.40***		5.42*	107.20***	228.04***	235.74***	321.30***
	Pec+Vis		1		10.40±0.18b	4.89±0.38	42.39±1.86b	7.20±0.23a	13.29±1.04b	7.80±0.67b
			2	9.55±0.06c	5.14±1.58	60.52±1.17a	1.91±0.10b	21.33±0.24a	19.67±0.00a
			3	21.24±0.33a	3.98±0.37	36.34±0.56c	7.64±1.21a	22.42±1.55a	20.40±1.06a
			F-value	2,620.46***	N.S	276.17***	59.79***	63.27***	288.65***
			Temperature (°C)	HP	Pec	45	22.85±0.70a	2.02±0.02b	63.74±1.12b	8.26±0.99a	27.35±1.22b	19.61±0.61b
	50					23.03±0.51a	4.11±0.86a	70.67±0.42a	1.61±0.12b	35.33±2.99a	21.86±0.08a
	55					20.55±0.47b	1.93±0.05b	63.90±0.79b	7.54±0.61a	27.35±0.53b	20.46±1.25ab
	F-value	17.85**				18.27**	64.41***	88.71***	17.87**	5.94*
	WB	Pec				45	16.27±0.29b	0.91±0.04b	63.84±0.60	8.10±0.98a	24.47±1.93b	20.74±0.23
				50	16.90±0.32a	4.92±1.17a	58.56±2.24b	1.86±0.10b	30.99±0.16a	21.23±0.01
				55	15.39±0.32c	0.95±0.20b	55.08±1.85c	7.53±0.23a	24.62±1.27b	21.60±0.62
F-value				17.81**	33.47**	19.94**	103.44***	23.25**	N.S
Pressure (MPa)				HP	Pec	50	16.58±0.68c3)	1.56±0.07b	62.52±0.67b	4.47±0.10c	30.03±0.41b	19.30±0.34c
	70	18.66±0.83b				1.21±0.03b	63.21±1.29b	3.59±0.07b	35.99±0.25a	20.89±0.10b
	100	23.03±0.51a				4.11±0.86a	70.67±0.42a	1.61±0.12a	35.33±2.99a	21.86±0.08a
	F-value	69.21***	30.04**			80.74***	645.52***	10.50*	114.28***

HP, high-pressure enzyme treatment; WB, water bath shaking with enzyme; Pec, pectinex ultra pulp; Pec+Vis, pectinex ultra pulp+viscozyme L (1 % enzyme on substrate 5 g).
RPSC, removal polysaccharide and starch content; SCPC, soluble crude polysaccharide content; WSI, water solubility index; WAI, water absorbance index; TS, total sugar; RS, reducing sugar.
All values are mean±SD (n=3).
Means with different small letters in the same column differ significantly by Duncan's multiple range test (p<0.05).
p<0.05
p<0.01
p<0.001
, not significant.