Table 6 Effect of high-pressure enzyme treatment on removal starch, crude polysaccharide and water solubility depend on enzyme type
Treatment | Enzyme | 2)RPSC (%) | SCPC (%) | WSI (%) | WAI (g/mL) | TS (%) | RS (%) |
1)CON | | e1.17±0.04e | 3.06±0.07 | d15.35±0.10e | c1.35±0.04d | f0.24±0.03d | g0.14±0.02f |
HP | Non-E | 3)0.99±0.01e | 5.33±1.53 | 15.90±0.41e | 1.46±0.07cd | 3.40±0.13c | 0.32±0.02f |
Cel | 22.37±0.23d | 3.58±1.52 | 64.72±1.23b | 2.13±0.15b | 41.67±1.96a | 18.90±0.11e |
Pec | 23.03±0.51c | 4.11±0.86 | 70.67±0.42a | 1.61±0.12c | 35.33±2.99b | 21.86±0.08d |
Vis | 24.12±0.23b | 4.42±0.15 | 58.81±0.59c | 2.07±0.09b | 42.14±0.28a | 26.77±1.08a |
Cel+Vis | 24.61±0.19a | 3.80±1.34 | 55.91±0.85d | 2.54±0.17a | 40.11±1.67a | 24.63±0.02c |
Pec+Vis | 23.99±0.16b | 3.99±0.87 | 59.66±0.41c | 2.16±0.07b | 39.23±1.96a | 25.80±0.55b |
F-value | 5,914.85*** | N.S | 3,615.38*** | 46.51*** | 373.31*** | 1,925.02*** |
WB | Non-E | E1.16±0.02 | 6.03±2.64 | D16.94±0.68 | C1.43±0.04 | E2.92±0.11 | F0.40±0.00 |
Cel | B15.02±0.20 | 5.05±1.25 | C54.65±2.06 | A2.12±0.32 | B30.79±0.37 | E18.93±0.30 |
Pec | A16.90±0.32 | 4.92±1.17 | AB58.56±2.24 | AB1.86±0.10 | B30.99±0.16 | C21.23±0.01 |
Vis | D9.55±0.06 | 4.81±2.19 | BC56.61±2.06 | B1.78±0.22 | C27.83±0.22 | B22.74±0.24 |
Cel+Vis | B14.77±0.04 | 6.43±1.47 | A59.71±1.49 | AB1.88±0.18 | A36.81±0.27 | A26.26±0.15 |
Pec+Vis | C12.38±0.36 | 5.14±1.58 | A60.52±1.17 | AB1.91±0.10 | D21.33±0.24 | D19.67±0.00 |
F-value | 3,188.42*** | N.S | 502.78*** | 7.69*** | 12,463.04*** | 13,820.64*** |
CON, 100°C reflex for 2 h; HP, high-pressure enzyme treatment (100 MPa, 50°C, 2 h); WB, water bath shaking with enzyme; Non-E, non-treatment enzyme; Cel, celluclast 1.5L FG; Pec, pectinex ultra pulp; Vis, viscozyme L; Cel+Vis, celluclast 1.5L FG+viscozyme L; Pec+Vis, pectinex ultra pulp+viscozyme L (1 % enzyme on substrate 5 g).
RPSC, removal polysaccharide and starch content; SCPC, soluble crude polysaccharide content; WSI, water solubility index; WAI, water absorbance index; TS, total sugar; RS, reducing sugar.
All values are mean±SD (n=3).
Means with different small letters in the same column differ significantly by Duncan's multiple range test (p<0.05).
Means with different capital letters in the same column (CON and WB) differ significantly by Duncan's multiple range test(p<0.05).
p<0.001
, not significant.