Table 6 Effect of high-pressure enzyme treatment on removal starch, crude polysaccharide and water solubility depend on enzyme type

Treatment Enzyme 2)RPSC (%) SCPC (%) WSI (%) WAI (g/mL) TS (%) RS (%)
1)CON e1.17±0.04e 3.06±0.07 d15.35±0.10e c1.35±0.04d f0.24±0.03d g0.14±0.02f
HP Non-E 3)0.99±0.01e 5.33±1.53 15.90±0.41e 1.46±0.07cd 3.40±0.13c 0.32±0.02f
Cel 22.37±0.23d 3.58±1.52 64.72±1.23b 2.13±0.15b 41.67±1.96a 18.90±0.11e
Pec 23.03±0.51c 4.11±0.86 70.67±0.42a 1.61±0.12c 35.33±2.99b 21.86±0.08d
Vis 24.12±0.23b 4.42±0.15 58.81±0.59c 2.07±0.09b 42.14±0.28a 26.77±1.08a
Cel+Vis 24.61±0.19a 3.80±1.34 55.91±0.85d 2.54±0.17a 40.11±1.67a 24.63±0.02c
Pec+Vis 23.99±0.16b 3.99±0.87 59.66±0.41c 2.16±0.07b 39.23±1.96a 25.80±0.55b
F-value 5,914.85*** N.S 3,615.38*** 46.51*** 373.31*** 1,925.02***
WB Non-E E1.16±0.02 6.03±2.64 D16.94±0.68 C1.43±0.04 E2.92±0.11 F0.40±0.00
Cel B15.02±0.20 5.05±1.25 C54.65±2.06 A2.12±0.32 B30.79±0.37 E18.93±0.30
Pec A16.90±0.32 4.92±1.17 AB58.56±2.24 AB1.86±0.10 B30.99±0.16 C21.23±0.01
Vis D9.55±0.06 4.81±2.19 BC56.61±2.06 B1.78±0.22 C27.83±0.22 B22.74±0.24
Cel+Vis B14.77±0.04 6.43±1.47 A59.71±1.49 AB1.88±0.18 A36.81±0.27 A26.26±0.15
Pec+Vis C12.38±0.36 5.14±1.58 A60.52±1.17 AB1.91±0.10 D21.33±0.24 D19.67±0.00
F-value 3,188.42*** N.S 502.78*** 7.69*** 12,463.04*** 13,820.64***
CON, 100°C reflex for 2 h; HP, high-pressure enzyme treatment (100 MPa, 50°C, 2 h); WB, water bath shaking with enzyme; Non-E, non-treatment enzyme; Cel, celluclast 1.5L FG; Pec, pectinex ultra pulp; Vis, viscozyme L; Cel+Vis, celluclast 1.5L FG+viscozyme L; Pec+Vis, pectinex ultra pulp+viscozyme L (1 % enzyme on substrate 5 g).
RPSC, removal polysaccharide and starch content; SCPC, soluble crude polysaccharide content; WSI, water solubility index; WAI, water absorbance index; TS, total sugar; RS, reducing sugar.
All values are mean±SD (n=3).
Means with different small letters in the same column differ significantly by Duncan's multiple range test (p<0.05).
Means with different capital letters in the same column (CON and WB) differ significantly by Duncan's multiple range test(p<0.05).
p<0.001
, not significant.