Table 2 Results of linearity validation of HPLC and UPLC methods (Linearity of silymarin analysis using HPLC and UPLC methods)

Parameter		Silychristin	Silydianin	Silybin A	Silybin B	Isosilybin A	Isosilybin B
HPLC method	Regression equation	y = 45119x + 9051	y = 44869x + 3037	y = 57429x + 2406	y = 57272x + 3276	y = 44100x + 3870	y = 82339x + 91
	Coefficient of determination (r2)	0.9999	0.9999	0.9999	0.9999	0.9999	0.9999
	LOD/LOQ (µg/mL)	0.0329/0.0998	0.2469/0.7482	0.0167/0.0506	0.0529/0.1602	0.0556/0.1686	0.0206/0.0623
UPLC method	Regression equation	y = 5339x + 288	y = 4620x + 174	y = 6482x + 159	y = 6626x + 301	y = 4745x + 174	y = 9172x + 140
	Coefficient of determination (r2)	0.9999	0.9999	0.9999	0.9999	0.9999	0.9999
	LOD/LOQ (µg/mL)	0.3568/1.0813	0.4267/1.2931	0.1566/0.4745	0.2071/0.6276	0.3920/1.1878	0.0544/0.1648