Table 1 Analytical conditions of HPLC and UPLC for the quantitative analysis of silymarin

Parameter	HPLC condition			UPLC condition
Column	YMC Pack Pro C18			Waters Acquity UPLC BEH C18
	(240 mm × 4.6 mm × 5 µm)			(50 mm × 2.1 mm × 1.7 µm)
Injection volume	20 µL			0.5 µL
Column oven temperature	35°C			30°C
Mobile phase	Min	Eluent A	Eluent B	Min	Eluent A	Eluent B
	0	85	15	0	85	15
	5	85	15	0.6	85	15
	20	55	45	3.6	55	45
	40	55	45	6	55	45
	41	85	15	6.2	85	15
	55	85	15	7	85	15
	Flow rate	1.0 mL/min			0.21 mL/min
Detection wavelength	288 nm			288 nm