Table 2 F-values for the particle size, pdI and zeta potential of peptide liposome by peptide concentration, manufacture scale and storage period

Particle size PdI Zeta potential

Primary homogenization condition Homogenizer speed 359.83*** 1.77 4.18*
Manufacture scale 392.93*** 0.29 33.73***
Homogenizer speed + Manufacture scale 11.28*** 0.99 2.96*

Secondary homogenization condition Ultrasonificatior power 10.93*** 2.29 18.18***
Manufacture scale 3255.50*** 35.62*** 57.02***
Ultrasonificatior power + Manufacture scale 5.44** 0.81 12.53***

Peptide concentration Peptide concentration 673.82*** 0.62 53.71***
Manufacture scale 555.56*** 0.72 1.97
Peptide concentration + Manufacture Scale 148.47*** 0.69 0.56
* p<0.05, ** p<0.01, *** p<0.001.