| Instrument | YL9100 HPLC |
| Column | Agilent 5 TC-C18(2) 250 × 4.6 mm |
| Column temperature | 30°C |
| Mobil phase | A - 10% ethanol + 0.1% phosphoric acid |
| B - 90% ethanol + 0.1% phosphoric acid | |
| Gradient condition | H2O/ qethanol linear gradient (from 20% |
| ethanol to 80% ethanol for 35 min) | |
| Flow rate | 1.0 mL/min |
| Injection volume | 20 micro liter |
| Detector wave length | 340 nm |