Table 2 The properties of nucleic acid amplification technology

Technology Target nucleic acid Polymerase Temperature Reference
PCR DNA, RNA Taq DNA polymerase Changed Saiki et al., 1988
Real-time PCR DNA, RNA Taq DNA polymerase Changed Heid et al., 1996
RCA DNA Phi29 DNA polymerase Isothermal Lizardi et al., 1998
LCR DNA Taq DNA polymerase Changed Barany, 1991
NASBA RNA T7 RNA polymerase Isothermal Kievits et al., 1991
LAMP DNA, RNA Bst DNA polymerase Isothermal Notomi et al., 2000