| No | Amino acids | Acidic extraction | Enzymatic extraction | Enzymatic & acidic extraction | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| | | | ||||||||
| 1 h | 3 h | 5 h | 1 h | 3 h | 5 h | 1 h | 3 h | 5 h | ||
| 1 | O-Phospho-L-serine | 0.3400±0.0004 | 0.4584±0.0001 | 0.4467±0.0004 | 0.1700±0.0001 | 0.1900±0.0001 | 0.1217±0.0001 | 0.1367±0.0001 | 0.0584±0.0401 | 0.0700±0.0066 |
| 2 | Taurine | - | - | - | - | - | - | - | - | 0.0034 |
| 3 | O-Phosphoethanolamine | - | - | - | - | - | - | - | 0.0017±0.0001 | 0.0050±0.0001 |
| 4 | Urea | 0.0184±0.0001 | 0.0100±0.0001 | 0.0167±0.0002 | 0.0267±0.0001 | 0.0100±0.0001 | 0.0384±0.0001 | 0.0117±0.0001 | 0.0050±0.0001 | 0.0100±0.0001 |
| 5 | L-Aspartic Acid | 0.2934±0.0011 | 0.6784±0.0089 | 0.7717±0.0025 | 0.3450±0.0001 | 1.7934±0.0251 | 3.6367±0.2933 | 0.5434±0.0491 | 1.7150±0.0970 | 3.1000±0.8062 |
| 6 | L-Threonine | 0.3600±0.0003 | 0.6417±0.0113 | 0.7700±0.0013 | 0.0100±0.0001 | 0.1084±0.0501 | 0.1550±0.0055 | 0.1080±0.0180 | 0.1850±0.0064 | 0.3317±0.0197 |
| 7 | L-Serine | 0.2984±0.0004 | 0.5267±0.0299 | 0.6417±0.0038 | 0.1417±0.0032 | 0.0650±0.0001 | 0.4400±0.0046 | 0.1817±0.0461 | 0.3350±0.0980 | 0.5917±0.0636 |
| 8 | L-Asparagine | 0.4334±0.00046 | 0.8367±0.0001 | 0.8400±0.0262 | 0.0217±0.0001 | 0.1350±0.0025 | 0.0800±0.0060 | 0.0517±0.0468 | 0.0467±0.0001 | 0.0300±0.0024 |
| 9 | L-Glutamic Acid | 1.9717±0.0520 | 3.5967±0.0049 | 3.7684±0.1336 | 0.0734±0.0001 | 0.7717±0.0011 | 1.1300±0.0899 | 0.8567±0.0594 | 1.4050±0.0849 | 2.3700±0.0959 |
| 10 | Theanine | 0.1384±0.0029 | 0.0517±0.0001 | 0.1017±0.0023 | 0.0167±0.0001 | 0.0784±0.0001 | 0.0484±0.0050 | 0.0567±0.0014 | 0.0984±0.0001 | 0.0400±0.0005 |
| 11 | D,L-α-Aminoadipic acid | 0.0600±0.0041 | 0.1067±0.0001 | 0.1184±0.0091 | 0.0317±0.0001 | 0.0900±0.0001 | 0.1234±0.0002 | 0.0834±0.0.38 | 0.1384±0.0095 | 0.1250±0.0262 |
| 12 | Glycine | 0.1184±0.0027 | 0.1834±0.02010 | 0.2250±0.0021 | 0.1117±0.0022 | 0.6317±0.0265 | 1.0350±0.0099 | 0.1750±0.0068 | 0.5667±0.0001 | 1.0617±0.0496 |
| 13 | L-Alanine | 0.5200±0.0121 | 0.8467±0.0050 | 1.0184±0.0011 | 0.0634±0.0013 | 0.0317±0.0001 | 0.0767±0.0010 | 0.1984±0.0049 | 0.4567±0.0956 | 0.8434±0.0055 |
| 14 | L-α-Amino-n-butyric acid | 0.0234±0.0001 | 0.0450±0.0001 | 0.0600±0.0005 | 0.0084±0.0001 | 0.0317±0.0001 | 0.0767±0.0001 | 0.0384±0.0001 | 0.0634±0.0001 | 0.1034±0.0001 |
| 15 | L-Valine | 0.4067±0.0001 | 0.7184±0.0226 | 0.8550±0.0001 | - | 0.0484±0.0001 | 0.0417±0.0001 | 0.0550±0.0001 | 0.0450±0.0001 | 0.1034±0.0001 |
| 16 | L-Cystine | 0.0850±0.0001 | - | 0.1984±0.0040 | - | - | - | 0.1817±0.0095 | 0.1817±0.0001 | 0.1500±0.0056 |
| 17 | L-Methionine | 0.4350±0.0072 | 0.7767±0.0078 | 0.8817±0.0096 | 0.0250±0.0050 | 0.2050±0.0562 | 0.2134±0.0001 | 0.1634±0.0001 | 0.2117±0.0001 | 0.3450±0.0405 |
| 18 | L-Isoleucine | 0.2984±0.0046 | 0.5300±0.0088 | 0.6100±0.0052 | 0.0134±0.0001 | 0.0167±0.0029 | 0.0867±0.0001 | 0.0167±0.0001 | 0.0317±0.0001 | 0.0267±0.0080 |
| 19 | L-Leucine | 2.4967±0.0411 | 4.0100±0.2107 | 4.2534±0.3106 | 0.0150±0.0001 | 0.4367±0.0064 | 0.3367±0.0001 | 0.4650±0.0094 | 0.5567±0.0001 | 0.8667±0.0965 |
| 20 | L-Tyrosine | 0.3617±0.0011 | 0.6584±0.0028 | 0.8434±0.0066 | 0.0267±0.0001 | 0.1517±0.0058 | 0.1367±0.0011 | 0.1334±0.0195 | 0.1767±0.0001 | 0.3384±0.0231 |
| 21 | L-Phenylalanine | 0.3800±0.0020 | 0.7617±0.0019 | 0.9717±0.0065 | 0.0050±0.0001 | 0.1334±0.0037 | 0.1334±0.0098 | 0.1067±0.0641 | 0.1600±0.0001 | 0.2767±0.0131 |
| 22 | b-Alanine | 0.2100±0.0042 | 0.3750±0.0083 | 0.4317±0.0001 | - | - | 0.0067±0.0076 | 0.0534±0.0010 | 0.0667±0.0025 | 0.0900±0.0401 |
| 23 | D,L-β-Aminoisobutyric acid | 1.4550±0.0705 | 2.4184±0.0816 | 2.6217±0.0356 | 0.0584±0.0001 | 0.3467±0.0200 | 0.3584±0.0040 | 0.4567±0.0001 | 0.4634±0.0950 | 0.5934±0.0495 |
| 24 | 4-Amino-n-butyric acid | 0.0734±0.0002 | 0.1434±0.0002 | 0.1200±0.0062 | 0.0234±0.0001 | 0.0634±0.0094 | 0.0567±0.0066 | 0.0150±0.0001 | 0.0350±0.0046 | 0.0550±0.0160 |
| 25 | L-Histidine | 0.2000±0.0590 | 0.3534±0.0181 | 0.3834±0.0861 | 0.0050±0.0001 | 0.0417±0.0011 | 0.0517±0.0010 | 0.0500±0.0026 | 0.0684±0.0001 | 0.1284±0.0462 |
| 26 | 1-Methyl-L-histidine | 0.0134±0.0078 | 0.0217±0.0.020 | 0.0300±0.0004 | - | 0.0034±0.0001 | 0.0200±0.0032 | 0.0084±0.0001 | 0.0167±0.0001 | 0.0250±0.0501 |
| 27 | 3-Methyl-L-histidine | - | 0.0100±0.0065 | - | - | 0.0034±0.0001 | - | 0.0067±0.0001 | 0.0100±0.0051 | 0.0200±0.0028 |
| 28 | L-Carnosine | 0.4167±0.0492 | 0.6734±0.0602 | 0.7800±0.0028 | 0.0517±0.0001 | 0.0884±0.0001 | 0.2034±0.0095 | 0.1250±0.0404 | 0.1167±0.0408 | 0.1750±0.0490 |
| 29 | L-Ornithine- monohydrochloride | 0.2550±0.0898 | 0.2784±0.0032 | 0.3767±0.0026 | - | 0.0250±0.0050 | 0.1100±0.0809 | 0.0434±0.0032 | 0.0617±0.0095 | 0.0884±0.0060 |
| 30 | L-Lysine | 0.2950±0.0195 | 0.5200±0.0001 | 0.5967±0.0048 | 0.0317±0.0001 | 0.0850±0.0150 | 0.2967±0.0019 | 0.1084±0.0068 | 0.2134±0.0001 | 0.4300±0.0048 |
| 31 | Ammonium Chloride | 0.0984±0.0048 | 0.1267±0.0084 | 0.1500±0.0062 | 0.3617±0.0658 | 0.4200±0.0615 | 0.3784±0.0094 | 0.2717±0.0025 | 0.2084±0.0001 | 0.2084±0.0064 |
| 32 | L-Arginine | 0.6167±0.0948 | 1.0767±0.1500 | 1.1467±0.0849 | 0.0984±0.0059 | 0.2584±0.0026 | 0.2917±0.0782 | 0.1884±0.0044 | 0.2867±0.0001 | 0.5784±0.0821 |
| Totals | 12.6717±0.9941 | 21.4334±0.8011 | 24.0284±0.0268 | 1.7350±0.0101 | 6.7117±0.0620 | 10.201±0.0907 | 4.8900±0.2051 | 7.9850±0.8021 | 13.1834±0.5201 | |