Table 1 Summary of purification of polyMG-specific lyase from Streptomyces violaceoruber

Step Total activity (Unit) Total protein (mg) Specific activity (Units/mg) Yield (%) Purification fold
Crude enzyme 399.0 1110.0 0.36 100 1
DEAE sephacel 203.5 130.2 1.56 51 4.4
SP Sepharose FF 174.4 12.0 14.59 43.7 40.6