| Parameter | Operation condition |
|---|---|
| Instrument | HPLC 1260 Infinities series |
| (Agilent Technology, Waldbronn, Germany) | |
| Column | ZORBAX Carbohydrate Analysis |
| (4.6 mm ID×250 mm, 5 μm) | |
| Mobile phase | A:B = 75:25(v:v) (A: Acetonitrile, B: D.W.) |
| Detector | RI |
| Column temp. | 35°C |
| Flow rate | 1 mL/min |
| Injection volume | 10 μL |
| Run time | 30 min |