Table 1.
Operating conditions of GC-MS in the analysis of polycosanol

Items Condition

Instrument GC: HP 5890(Agilent Inc., Santa Clara, CA, USA) MS: HP 5973(Agilent Inc., Santa Clara, CA, USA)
Column DB-1MS, fused silica capillary column 30 m (length), 0.32 mm (i. d), 0.25 µm (film thick.) 0.25 µm (film thickness)
Transfer temp. 250°C
Injector temp. 320°C
Flow rate Helium 5 mL/min
Column temp. Programmed from 230 to 305°C, 3°C/min 305 to 320°C, 20°C/min
MS quad. temp. 150°C
MS source temp. 230°C
Ionization EI (70 eV)