| Column | Zorbax ODS C18 (4.6 mm×150 mm, 5 µm) | Zolbax ODS C18 (4.6 mm×150 mm,5 µm) | J’ spere ODS H-80 (4.6 mm×250 mm,4 µm) |
| Detector | Fluorescence detector (FLD, JASCO, Tokyo, Japan) Ex=225 nm, Em+295 nm | Differential refractive index detector(RID) | Evaporative light scattering detector(ELSD) Temperature : 90°C;Gas: nitrogen) |
| Mobile phase | A:B = 2:8 (A: methanol, B: water) | A:B = 8:2 (A: methanol added 0.25M sodium perchlorate, B: water) | A:B=7:3 (A: methanol, B: water) |
| Flow rate | 1.0 mL/min | 1.5 mL/min | 0.8 mL/min |
| Column temperature | 25°C | 25°C | 25°C |
| Injection volume | 20 uL | 20 uL | 20 uL |