Table 2 Degree of hydrolysis (DH, %) of enzymatic hydrolysates at various reaction time using commercial proteases under ambient pressure and 100 MPa at 50°C

Reaction time (h)	Degree of hydrolysis (%)

	Control1)	Alcalase^® 2.4 L	Flavourzyme^TM 500 MG	Neutrase^®	Protamex^TM	Mixed enzyme2)

0.1 MPa
1	2.0±0.4^a,3)	14.9±0.5^a	15.7±1.0^a	9.3±1.2^a	11.2±1.8^a	21.4±0.4^a
2	7.7±1.3^b	20.1±1.0^b	22.9±0.1^b	13.8±1.3^b	14.5±2.2^ab	23.4±0.9^ab
6	13.8±0.3^c	23.2±0.6^bc	27.2±1.2^bc	18.7±1.6^c	17.1±1.9^b	26.1±2.7^bc
12	18.1±0.4^cd	25.6±0.7^cd	29.5±0.5^c	20.9±1.6^cd	19.5±1.0^bc	30.2±0.9^cd
24	22.1±1.4^d	28.9±0.7^d	31.4±0.6^cd	24.1±2.0^d	22.6±2.1^c	32.2±0.7^de
100 MPa
1	19.5±4.1^d	33.3±2.4^e	36.8±5.3^de	32.9±1.7^e	33.3±0.2^d	36.2±1.8^ef
2	18.7±2.6^cd	34.9±3.0^e	39.4±5.4^e	33.7±1.5^e	34.8±2.3^d	37.8±2.5^f
6	32.8±3.7^e	43.3±3.2^f	46.9±3.0^f	42.9±2.7^f	42.9±5.2^e	45.0±4.7^g
12	42.1±5.9^f	52.7±1.9^g	57.4±6.3^g	50.9±3.4^g	51.9±4.9^f	49.0±3.3^g
24	47.1±1.8^g	58.3±3.5^h	62.9±4.2^g	59.5±3.2^h	57.2±2.7^g	57.5±2.8^h

¹⁾Control was hydrolyzed without any commercial protease
²⁾Mixed enzyme (ME): Alcalase^® 2.4 L : Flavourzyme^TM 500 MG=1:5
³⁾Mean±SD (n=3)
^a-hMeans within the same column with different superscripts are significantly different at p<0.05.