Table 2.
Operating conditions of LC-M S/MS for the analysis of amino acid sequences.

Items	Condition
Instrument	Quadrupole-TOF LC-MS/MS spectrometer (Q-Star Elite, Applied Biosystems)
Column	1. Agilent Zorbax 300SB-C18 column (300 µm i.d×50 mm, 5 µm, 100Å, Agilent Technologies)
	2. Agilent zorbax 300SB-C18 column (75 µm i.d×150 mm, 3.5 µm, 100Å, Agilent Technologies)
Ionization	Ion spray voltage(2300eV)
Flow rate	300 µL/min
Mobile phase	Eluent A - 30% acetonitrile
Injection volumn	1-2 µL