| Samples | Free amino acids |
|---|---|
| Instruments | Younglin Acme 2000 |
| (Younglin Co., Anyang, Korea) | |
| Column | Waters AccQ-Tag column |
| Column oven | 37°C |
| Detector | UV detector |
| Absorbance | 254 nm |
| Flow rate | 1.0 mL/m |
| Mobile phase | Eluent A - AccQ-Taq |
| buffer | |
| Eluent B - Acetonitrile | |
| Eluent C - Water | |
| Injection volumn | 10 L |