| Parameter | Analytical Conditions |
|---|---|
| HPLC | Waters 2690 |
| Column | Shodex Asahipak NH2P-50 4E (300 mm × 3.9 nm, 10 µm) |
| Flow rate | 1.0 mL / min |
| Injection volume | 20 µL |
| Detector | Waters 410 (RI Detector ) |
| Column temperature | 30°C |
| Mobile phase | Acetonitrile : H2O (75:25, v/v) |