Table 2.
Changes in amino acids of beef-bone broth with extraction time (unit: mg/kg).

Sample	Aspartate	Glutamate	Serine	Histidine	Glycine	Treonine	Arginine	Alanine	GABA
3 h	1107.25	2742.91	1100.48	352.22	7224.11	587.19	2588.48	2962.46	63.73
5 h	2077.11	4150.71	1660.10	474.07	10878.21	899.75	3996.62	4535.04	53.87
8 h	2043.92	4143.72	1606.82	516.03	10682.14	868.50	3879.45	4490.28	76.10
11 h	2136.99	4382.11	1655.17	513.08	11099.27	935.32	4013.01	4627.28	66.64
15 h	2387.76	4696.54	1795.36	559.68	12184.48	932.58	4349.50	5120.22	70.16
Tyrosine	Valine	Methionine	Phenylalanine	Isoleucine	Leucine	Lysine	Hydroxy proline	Proline	Total Amino acid
221.05	806.59	139.72	556.35	454.92	1025.82	939.48	4014.42	3901.73	30788.92
353.07	1212.37	308.94	957.83	736.46	1637.55	1442.28	5946.90	6086.25	47407.13
378.98	1241.66	328.27	974.33	751.27	1686.63	1694.05	5878.08	6351.60	47591.83
367.48	1264.04	386.00	1013.50	777.62	1764.99	1729.86	5731.90	6147.28	48611.56
470.11	1384.92	413.36	1125.13	858.45	1919.66	1853.27	6705.63	7402.13	54228.94