| System | Agilent 1200LC |
| Column | C18 column (4.6 mm×150 mm, 5 μm) |
| Detector | FL Detector : Emission 450 nm, Excitation |
| 340 nm(OPA) | |
| Emission 305 nm, Excitation 266 nm(FMOC) | |
| UV Detector : 338 nm | |
| Flow rate | 1.5 mL/min |
| Mobile phase | A : 20 mM sodium phosphate monobasic, pH 7.8 |
| B : 3 DW / acetonitrile / methanol | |
| (10 : 45 : 45 %(v/v)) | |
| Injection Volume | 0.5 µL |
| Column Temperature | 40°C |
| Sample Temperature | 20°C |