Article
HPLC를 이용한 주방세제의 음이온 및 비이온 계면활성제 분석
김장미1, 오남순1, 김경태2, 문경환3, 박세종4, 신동우4, 김미혜4, 김진만1,*
Analysis of Dish Detergent in Anionic and Nonionic Surfactants by HPLC
Jang-Mi Kim1, Jung-Min Park1, Kyung-Tae Kim2, Kyong-Whan Moon3, Se-Jong Park4, Dong-Woo Shin4, Mee-Hye Kim4, Jin-Man Kim1,*
1Department of Food Science and Biotechnology of Animal Resources, Konkuk University
2Research and Development Institute, Daesung C&S Co., Ltd.
3Department of Environment and Health, Korea University
4Food Additives and Packages Division, Ministry of Food and Safety
*Corresponding author: Jin-Man Kim, Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143- 701, Korea. Tel: +82-2-450-3688; Fax: +82-2-455-1044, E-mail:
jinmkim@konkuk.ac.kr
ⓒ Copyright 2013 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: Mar 08, 2013; Revised: Apr 22, 2013; Accepted: Apr 24, 2013
Published Online: May 31, 2013
Abatract
This study was carried out to develop an analysis method for the determination of anionic and nonionic surfactants. The condition for simultaneous analysis of anionic and nonionic surfactants by high-performance liquid chromatography (HPLC) equipped with an evaporated light scattering detector (ELSD) was investigated. Alkyl polyglucoside (APG) and alpha olefin sulfonate (AOS) were analyzed on a C18-boned silica column with a methanol and water gradient condition by HPLC/ELSD. Calibration curves were linear in the range of 100-500 mg/L on APG and 500- 4000 mg/L on AOS, with a coefficient of determination (r2) of 0.9999. The limits of detection (LOD) for APG and AOS were 2.29 and 16.55 mg/L, and the limits of quantification (LOQ) were 7.63 and 55.16 mg/L, respectively. The recoveries of the surfactants were 99.29% on APG and 96.11% on AOS. The established method provided acceptable precision and accuracy. Our methods would be useful for the detection of APG and AOS in dishwashing detergents.
Keywords: surfactant; high-performance liquid chromatography; alpha olefin sulfonate; alkyl polyglucoside microanalysis
