알로에 베라 겔 한외여과 다당분획의 분자적 특성

권혜미¹, 백진홍², 차지민², 이신영¹^,^*

Molecular Properties of Polysaccharide Rich Fraction from Ultra-filtration of Aloe vera Gel

Hye Mi Kwon¹, Jin Hong Beak², Ji Min Cha², Shin Young Lee¹^,^*

¹강원대학교생물공학과

²KJM 알로에 R&D 센터

¹Department of Bioengineering and Technology, Kangwon National University

²KJM Aloe R&D Center

^*Corresponding author: Shin-Young Lee, Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200-701, Korea. Tel: +82-33-250-6273; Fax: +82-33-243-6350, E-mail: sylee@kangwon.ac.kr

ⓒ Copyright 2013 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Sep 28, 2012; Revised: Dec 10, 2012; Accepted: Dec 10, 2012

Published Online: Feb 28, 2013

Abatract

Two fractions (permeate and retentate) from fiber-free Aloe vera gel were separated using ultra-filtration (UF) system with tubular ceramic membrane (MWCO of 50 kDa), and their molecular properties were investigated. The retentate of UF Aloe gel was a polysaccharide-rich fraction containing about 2.3-fold higher polysaccharide content than fiber-free gel. The FT-IR and ¹H NMR spectra of this fraction showed the characteristic patterns of β-binding polysaccharide and a higher degree of acetylation indicating a higher level of bioactive polysaccharide content. The molecular weight and polydispersity of polysaccharide fraction from GPC (gel permeation chromatography) were determined to be 36.8-43.9 kDa and 1.24, respectively, indicating a kind of undispersed polysaccharide. From the SEM observation, the surface structure of polysaccharide fraction had a gel cluster-like structure with a convoluted rough surface. The molecular conformation by Congo red assay exhibited a property of helix structure confirming the existence of a higher-ordered structure as a biological activity conformation.

Keywords: Aloe vera gel; ultra-filtration fraction; polysaccharide; molecular property