고체평판배지에서의 홍국균의 배양, monacolin K 및 색소생산 특성

김경선¹, 안준배², 김창섭³, 박윤제¹^,^*

Characteristics of growth, monacolin K and pigment production by Monascus strains on plate culture

Kyungsun Kim¹, Junbae Ahn², Chang Sup Kim³, Youn-Je Park¹^,^*

¹차의과학대학교 바이오공학과

²서원대학교 외식산업학과

³한밭대학교 화학생명공학과

¹Department of Applied Bioscience, CHA University

²Department of Food Service Industry, Seowon University

³Department of Chemical and Biological Engineering, Hanbat National University

^*Corresponding author: Youn-Je Park, Department of Applied Bioscience, College of Life Science, CHA University, 502 Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-840, Korea. Tel: +82-31-8017-9464; Fax: +82-31-8017-9464, E-mail: yjpark@cha.ac.kr

ⓒ Copyright 2012 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 23, 2012; Revised: Oct 31, 2012; Accepted: Nov 05, 2012

Published Online: Nov 30, 2012

Abatract

The production of color pigments and monacolin K by Monascus strains were investigated on agar plates while monitoring cell growth patterns. Thirty five different strains were collected and cultured on potato dextrose yeast extract agar (PDYA), potato dextrose agar (PDA) and malt extract agar medea (MEA) at two different incubation temperature conditions, 25°C and 30°C. The growth rates of most of strains were highest on PDYA medium, and hyphal growth was faster at 30°C than at 25°C. Both red and yellow pigments were highly produced by high pigment-producing strains on agar plate cultures. High pigment-producing strains produced less amount of monacolin K while low pigment- producing strains produced much more monacolin K. Any citrinin was not detected from the monacolin K-producing strains. These results imply that the selection of low pigment-producing strains cultured on agar plates could be applied for primary screening of Monascus strains for preparation of red mold rice.

Keywords: Monascus; plate culture; growth rate; monacolin K; pigment