Aloe vera L. Callus의 현탁배양에 의한 세포외 다당생산 및 최적화

김명욱¹, 조영제², 이신영³^,^*

Production and Optimization of Extracellular Polysaccharide by Suspension Cultivation of Aloe vera L. Callus

Myung-Uk Kim¹, Young-Je Cho², Shin-Young Lee³^,^*

¹(재)경북해양바이오산업연구원

²경북대학교 식품공학부

³강원대학교 생물공학과

¹Gyeongbuk Institute for Marine Bioindustry

²School of Food Science, Kyungpook National University

³Department of Bioengineering and Technology, Kangwon National University

^*Corresponding author: Shin-Young Lee, Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200- 701, Korea. Tel: +82-33-250-6273; Fax: +82-33-243-6350, E-mail: sylee@kangwon.ac.kr

ⓒ Copyright 2012 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 23, 2012; Revised: Aug 22, 2012; Accepted: Aug 23, 2012

Published Online: Aug 31, 2012

Abatract

The extracellular polysaccharide production from suspension culture of Aloe vera L. was analyzed, and the optimization for suspension cultivation was investigated. From specific polysaccharide assay of glucomannan, FT-IR and ¹H NMR spectrum, the extracellular polysacchride from suspension culture was found to be similar to β-1,4-glucomannan originated from Aloe vera fresh leaf. The polysaccharide existed in an acetylated form and its molecular weight by gel permeation chromatography was estimated to be 490 kD (Mn = 440 kD; polydispersity = 1.115). From medium optimization by simplex-cemtroid design using MINITAB^®, the callus growth on the 2,4-D of 11.82 μM, kinetin of 13.84 μM and α-naphthalene acetic acid of 4.34 μM was turned out to be significantly superior than the growth on the other culture media combinations. Under this optimal culture media, other conditions such as the suspension culture's temperature, pH and the growth terms were determined. These results showed that the callus growth was optimum at 25°C, pH 5.5 and in 2 weeks. The maximum levels of growth and extracellular polysaccharide production of callus by optimal conditions were about 20.4 and 2.5 g/L, respectively, showing the profile of extracellular polysaccharide production was closely related to the callus growth.

Keywords: Aloe vera L.; suspension culture; extracellular polysaccharide; optimization; mixture experiment