흑미 미강의 기능성 성분 추출 공정 최적화

조인희¹, 최용희²^,^*

Optimization of Extraction of Functional Components from Black Rice Bran

In-Hee Jo¹, Yong-Hee Choi²^,^*

¹경북대학교 식품공학과

²경북대학교 식품생물산업연구소

¹Department of Food Science and Technology, Kyungpook National University

²Food & Bio-Industry Research Institute, Kyungpook National University

^*Corresponding author: Yong-Hee Choi, Department of Food Science and Technology, College of Agriculture and Life and Science, Kyungpook National University, 1370 Sangyoek-dong, Buk-gu, Daegu, 702-701, Korea, Tel: +82-53-950-5777; Fax: +82-53-950-6772, E-mail: yhechoi@knu.ac.kr

ⓒ Copyright 2011 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 13, 2011; Revised: Nov 14, 2011; Accepted: Nov 14, 2011

Published Online: Nov 30, 2011

Abatract

The purpose of this study was to determine the optimum ethanol extraction conditions for maximum extraction of functional components such as ferulic acid, oryzanol, and tocopherol from black rice bran using Response Surface Methodology (RSM). A central composite design was applied to investigate the effects of the independent variables of solvent ratio (X₁), extraction temperature (X₂) and extraction time (X₃) on the dependent variables such as total phenol components (Y₁), total flavonoids compounds (Y₂), electron donating ability (Y₃), γ-oryzanol (Y₄), ferulic acid (Y₅) and α-tocopherol components (Y₆). ANOVA results showed that coefficients of determination (R-square) of estimated models for dependent variables ranged from 0.8939 to 0.9470. It was found that solvent ratio and extraction temperature were the main effective factors in this extraction process. Particularly, the extraction efficiency of ferulic acid, γ-oryzanol and α-tocopherol components were significantly affected by extraction temperature. As a result, optimum extraction conditions were 20.35 mL/g of solvent ratio, 79.4°C of extraction temperature and 2.88 hr of extraction time. Predicted values at the optimized conditions were acceptable when compared with experimental values.

Keywords: black rice bran; ferulic acid; γ-oryzanol; optimization; ethanol extraction