Article
탈지미강으로부터 Phytic Acid의 추출과 정제의 최적화
최문실1, 한복경1, 최혁준1, 박영서2,*
Optimization of Extraction and Purification of Phytic Acid from Defatted Rice Bran
Moon Sil Choi1, Hyuk Joon Choi1, Bok Kyung Han1, Young-Seo Park2,*
1Research & Development Department, BKbio Co., LTD.
2Department of Food Science & Biotechnology, Kyungwon University
*Corresponding author: Young-Seo Park, Department of Food Science and Biotechnology, Kyungwon University, San 65 Bokjeong-dong, Sujeong-gu, Seongnam, 461-701, Korea, Tel: +82-31-750-5378; Fax: +82-31-750-5273, E-mail:
ypark@kyungwon.ac.kr
ⓒ Copyright 2011 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received: Aug 06, 2011; Revised: Aug 16, 2011; Accepted: Aug 17, 2011
Published Online: Aug 31, 2011
Abatract
The optimum condition for the extraction and purification processes of phytic acid from defatted rice bran was examined. The phytic acid was efficiently extracted when the defatted rice bran was treated with 10 volumes of 0.5% HCl for 1 hr. For the neutralization of acid-treated extract, 0.5% NaOH was the most acceptable. To purify phytic acid, Diaion HP20 resin was used to remove impurities from the extract. The flow-through was then loaded onto ion exchange columns packed with various resins and among them, Amberlite IRA-416 resin showed highest recovery yield. When the phytic acid was absorbed onto Amberlite IRA-416 resin and then eluted with 0.5% NaOH, 89% of applied phytic acid was eluted. Most proteins were removed from the purified phytic acid and total protein content of the phytic acid was 0.14% (w/w).
Keywords: phytic acid; defatted rice bran; extraction; ion exchange column chromatography
