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Food Engineering Progress
Korean Society for Food Engineering

Food Eng. Prog. 2011; 15(1):6-14

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2011.15.1.6

Article

흰목이와 흑목이 버섯의 다당추출 및 유리라디칼 소거활성 비교

김현민1, 허원1, 이신영1,*
Polysaccharide Extraction and Comparison of Free Radical Scavenging Activities from Tremella fuciformis and Auricularia auricula Fruit Body
Hyeon-Min Kim1, Won Hur1, Shin-Young Lee1,*
1강원대학교 생물공학과
1Department of Bioengineering and Technology, Kangwon National University
*Corresponding author: Shin-Young Lee, Department of Bioengineering and Technology, Kangwon National University, Chuncheon 200-701, Korea, Tel: +82-33-250-6273; Fax: +82-33-243-6350, E-mail: sylee@kangwon.ac.kr

ⓒ Copyright 2011 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Sep 06, 2010; Revised: Dec 30, 2010; Accepted: Dec 31, 2010

Published Online: Feb 28, 2011

Abatract

The polysaccharides from fruit body of Auricularia auricula and Tremella fuciformis were extracted using hot water, and partially purified through ethanol precipitation and dialysis. Free radical scavenging activities of the crude and purified polysaccharides were examined and compared each other. Free radical scavenging activities of the partially purified polysaccharides were higher than those of crude polysaccharides. DPPH free radical, ABTS radical and SOD-like activities of partially purified polysaccharide at 1 mg/mL of concentration from A. auricula were 61.7, 9.6 and 38.9%, respectively, while those of T. fuciformis were 9.6, 5.7 and 15.3%, respectively. Results of site and nonsite specific hydroxyl radical scavenging activities indicated that the partially purified polysaccharide fractions from A. auricula and T. fuciformis exhibited the hydroxyl radical scavenging effect by hydrogen donating ability and iron ion chelating ability. Also, reducing powers of A. auricula and T. fuciformis were 77.1 and 14.7% of BHT (0.1%) as standard, respectively. It was suggested that antioxidant activities of A. auricula were about 1.4~6.4 times higher than those of T. fuciformis due to different levels of polyphenol content.

Keywords: A. auricula; T. fuciformis; crude and purified polysaccharide; free radical scavenging activity