탈지미강 단백질의 가수분해 및 분해물의 특성 연구

김창원¹, 김현석², 김병용¹, 백무열¹^,^*

Proteolysis of Defatted Rice Bran Using Commercial Proteases and Characterization of Its Hydrolysates

Chang-Won Kim¹, Hyun-Seok Kim², Byung-Yong Kim¹, Moo-Yeol Baik¹^,^*

¹경희대학교 생명자원과학연구원, 생명과학대학 식품공학과

²안동대학교 식품생명공학과

¹Department of Food Science and Biotechnology, Institute of Life Science Resources, Kyung Hee University

²Department of Food Science & Biotechnology, Andong National University

^*Corresponding author: Moo-Yeol Baik, Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Korea, Tel: +82-31-201-2625; Fax: +82-31-204-8116, E-mail: mooyeol@khu.ac.kr

ⓒ Copyright 2011 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 16, 2010; Revised: Feb 10, 2011; Accepted: Feb 11, 2011

Published Online: Feb 28, 2011

Abatract

The defatted rice bran (DRB) was enzymatically hydrolyzed using eight commercial proteases for 4hr at optimum pH and temperature. Proteolytic hydrolysates were examined in supernatant and precipitate using lowry, semimicro kjeldahl and gravimetric method using weight difference before and after enzymatic hydrolysis. In lowry and kjeldahl protein assay method, two proteases (Alcalase and Protease N) were found to be the most effective enzymes. In gravimetric method, 60.6~118.3 mg protein/g DRB was hydrolyzed after eight commercial proteases treatments. Similar to lowry and kjeldahl method, 118.3 and 107.1 mg protein/g DRB were hydrolyzed after Alcalase and Protease N treatments, respectively. When two or three effective proteases (Protamex, Alcalase and Protease N) were applied at one time to obtain synergistic effect, significant increase (P<0.05) was observed when three proteases were applied at one time (63.4 mg protein/g DRB in lowry method and 204.5 mg protein/g DRB in gravimetric method). This result suggests that Alcalase and Protease N were the most effective enzymes for proteolysis of DRB and three commercial enzymes (Protamex, Alcalase and Protease N) showed the synergistic effect on the hydrolysis of DRB.

Keywords: defatted rice bran; rice protein; protease; enzymatic hydrolysis