Xylanase를 생산하는 호알칼리성 균주의 분리 및 동정

최지휘¹, 배동훈¹^,^*

Isolation and Identification of Alkalophilic Microorganism Producing Xylanase

Ji-Hwi Choi¹, Dong-Hoon Bai¹^,^*

¹단국대학교 식품공학과

¹Department of Food Engineering, Dankook University

^*Corresponding author: Dong-Hoon Bai, Department of Food Engineering, Dankook University, Cheonan 330-714, Korea, Tel: +82-41-550-3562; Fax: +82-41-550-3566, E-mail: baidh@dankook.ac.kr

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jul 8, 2010; Revised: Aug 16, 2010; Accepted: Aug 18, 2010

Published Online: Aug 31, 2010

Abatract

An alkalophilic microorganism named DK-2386, which produces xylanase, was isolated from soil of Taejo-mountain, Cheonan-si, Chungnam, Korea. The isolated strain was characterized as Gram-positive, with size of 0.4×2.5 μm, spore forming, anaerobic, catalase positive, possessed with hydrolysis abilities of casein, starch, sodium carboxy methyl cellulose, and xylan, reduction of nitrate to nitrite, resistant against lysozyme, urease positive, and motility positive. The color of culture broth was reddish yellow. The strain DK-2386 was identified as Bacillus agaradhaerens by whole cell fatty-acid composition analysis and 16S rDNA sequence analysis. However, it was not identical to Bacillus agaradhaerens 40952 obtained from the Korean Culture Center of Microorganism in its colour of culture broth. Therefore, we have named the newly isolated strain as Bacillus agaradhaerens DK-2386.

Keywords: Bacillus agaradhaerens; alkalophiles; xylanase