Food Engineering Progress
Korean Society for Food Engineering
Article

Sourdough에서 분리된 유산균의 고농도 배양을 위한 중화제로서 Calcium Carbonate-Alginate Bead의 이용가능성 평가

정승원1, 이광근1, 김철우2, 이수한3,*
Seung-Won Jung1, Kwang-Geun Lee1, Cheol Woo Kim2, Su Han Lee3,*
1동국대학교 식품생명공학과
2한국식품공업협회
3을지대학교 식품산업외식학과
1Department of Food Science & Technology, Dongguk University
2Korea Advanced Food Research Institute
3Department of Food Technology and Services, Eulji University
*Corresponding author: Su Han Lee, Professor, Department of Food Technology and Services, Eulji University, 212, Yanggi-dong, Sujeonggu, Seongnam-shi, Gyeonggi-do 461-713, Korea, Tel: +82-31-740-7196; Fax: +82-31-740-7349, E-mail: shlee@eulji.ac.kr

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 28, 2010; Revised: Jul 02, 2010; Accepted: Jul 05, 2010

Published Online: Aug 31, 2010

Abatract

Lab scale experiments were conducted in order to assess the applicability of CaCO3-alginate beads as neutralizer for the high cell density cultivation and prepare the direct vat inoculation cultures of isolated sourdough lactic acid bacteria. With increasing the amount of bead and decreasing the diameter of bead in acidic solution, the neutralizing effect of CaCO3-alginate bead became higher. In batch process with CaCO3-alginate beads, Lactobacillus amylovorus DU-21 isolated from sourdough showed the highest viable cell counts and optical density in MRS broth. The values of viable cell counts and optical density were 9.996 log CFU/mL and 3.97, respectively. Experiments on the conditions which increase viability during lyophilization were carried out and the following results were obtained; 15% glycerol revealed the high cryoprotective effect on the concentrated cultures during lyophilization among the two cryoprotective agents. Consequently, CaCO3-alginate beads and 15% glycerol were found to be useful not only to cultivate Lactobacillus amylovorus DU-21 but also to preserve strain.

Keywords: CaCO3-alginate beads; neutralizer; direct vat inoculation cultures; lactic acid bacteria