Sourdough에서 분리된 유산균의 고농도 배양을 위한 중화제로서 Calcium Carbonate-Alginate Bead의 이용가능성 평가

정승원¹, 이광근¹, 김철우², 이수한³^,^*

Assessment of Applicability of a Calcium Carbonate-Alginate Beads as Neutralizer for the High Cell Density Cultivation of Isolated Sourdough Lactic Acid Bacteria

Seung-Won Jung¹, Kwang-Geun Lee¹, Cheol Woo Kim², Su Han Lee³^,^*

¹동국대학교 식품생명공학과

²한국식품공업협회

³을지대학교 식품산업외식학과

¹Department of Food Science & Technology, Dongguk University

²Korea Advanced Food Research Institute

³Department of Food Technology and Services, Eulji University

^*Corresponding author: Su Han Lee, Professor, Department of Food Technology and Services, Eulji University, 212, Yanggi-dong, Sujeonggu, Seongnam-shi, Gyeonggi-do 461-713, Korea, Tel: +82-31-740-7196; Fax: +82-31-740-7349, E-mail: shlee@eulji.ac.kr

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 28, 2010; Revised: Jul 02, 2010; Accepted: Jul 05, 2010

Published Online: Aug 31, 2010

Abatract

Lab scale experiments were conducted in order to assess the applicability of CaCO₃-alginate beads as neutralizer for the high cell density cultivation and prepare the direct vat inoculation cultures of isolated sourdough lactic acid bacteria. With increasing the amount of bead and decreasing the diameter of bead in acidic solution, the neutralizing effect of CaCO₃-alginate bead became higher. In batch process with CaCO₃-alginate beads, Lactobacillus amylovorus DU-21 isolated from sourdough showed the highest viable cell counts and optical density in MRS broth. The values of viable cell counts and optical density were 9.996 log CFU/mL and 3.97, respectively. Experiments on the conditions which increase viability during lyophilization were carried out and the following results were obtained; 15% glycerol revealed the high cryoprotective effect on the concentrated cultures during lyophilization among the two cryoprotective agents. Consequently, CaCO₃-alginate beads and 15% glycerol were found to be useful not only to cultivate Lactobacillus amylovorus DU-21 but also to preserve strain.

Keywords: CaCO₃-alginate beads; neutralizer; direct vat inoculation cultures; lactic acid bacteria