Glass Slide-based Immunosensing for C-Reactive Protein Using Quantum Dot-Antibody Conjugate

Namsoo Kim¹^,^*, Sun Mi Oh¹, Chong-Tai Kim¹, Yong Jin Cho¹

¹Food Bio-nano Technology Research Group, Korea Food Research Institute

^*Corresponding author: Namsoo Kim, Food Bio-nano Technology Research Group, Korea Food Research Institute, Seongnam, Gyeonggi 463-746, Republic of Korea, Tel: +82-31-780-9131; Fax: +82-31-709-9876, E-mail : k9130sen@hanmail.net

ⓒ Copyright 2010 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 07, 2009; Revised: Nov 19, 2009; Revised: Feb 03, 2010; Accepted: Feb 04, 2010

Published Online: Feb 28, 2010

Abatract

C-Reactive protein (CRP), which is an 118 kDa pentameric protein, was secreted by the liver is an important biomarker for coronary disease, hypertension and inflammation. In this study, a method for CRP detection exploiting quantum dot (Qdot)-antibody conjugate was developed according to an indirect-competitive immunosensing protocol. For this purpose, a streptavidin-bound Qdot₆₀₅ was linked with a separately prepared biotinylated monoclonal antirat CRP antibody to produce a Qdot-antibody conjugate. The immunosensing was performed at 0.1 and 20 nM of the coating antigen and conjugate, respectively. The current method was found very sensitive in CRP detection, judging from the concentration-dependent fluorescence emission.

Keywords: glass slide; immunosensing; C-reactive protein; quantum dot-antibody conjugate