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Food Engineering Progress
Korean Society for Food Engineering

Food Eng. Prog. 2009; 13(4):326-334

pISSN: 1226-4768, eISSN: 2288-1247

DOI: https://doi.org/10.13050/foodengprog.2009.13.4.326

Article

목이버섯 추출물로부터 항혈전물질의 정제와 항혈전효과

박영서1,*, 최혁준2, 최태현3
Purification of Antithrombotic Material from Auricularia auricular-judae Extracts and Its Antithrombotic Activity
Young-Seo Park1,*, Hyuk-Joon Choi2, Tae Hyun Choi3
1경원대학교 식품생물공학과
2(주)비케이바이오
3한국원자력의학원
1Department of Food Science and Biotechnology, Kyungwon University
2BK bio Co., Ltd.
3Korea Institute of Radiological and Medical Sciences
*Corresponding author: Young-Seo Park, Department of Food Science and Biotechnology, Kyungwon University, Seongnam, Gyeonggi-do 461-701, Korea, Tel: +82-31-750-5378; Fax: +82-31-750-5273, E-mail: ypark@kyungwon.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Oct 26, 2009; Revised: Nov 17, 2009; Accepted: Nov 17, 2009

Published Online: Nov 30, 2009

Abatract

Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.

Keywords: Auricularia auricular-judae; anticoagulant activity; polysaccharide