알로에 겔의 in vitro 생리기능 특성에 미치는 삼투탈수공정의 영향

김성아¹, 백진홍¹, 이신영²^,^*

Effect of DIS Process on in vitro Physiological Functionality of Aloe vera Gel

Sung-A Kim¹, Jin-Hong Baek¹, Shin-Young Lee²^,^*

¹주)김정문 알로에 과학연구소

²강원대학교 생물공학과

¹KJM Aloe R&D Center

²Department of Bioengineering and Technology, Kangwon National University

^*Corresponding author: Shin-Young Lee, Department of Bioengineering and Technology, Kangwon National University, Chunchon 200-701, Korea, Tel: +82-33-250-6273; Fax: +82-33-243-6350, E-mail: sylee@kangwon.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Aug 12, 2009; Revised: Oct 21, 2009; Accepted: Oct 21, 2009

Published Online: Nov 30, 2009

Abatract

In vitro physiological functions such as jack bean (Canavalia ensiformis) urease inhibitory activity and retarding effect of glucose/bile acid of Aloe vera gel concentrated by the optimized DIS (Dewatering Impregnation & Soaking) process conditions were examined. Urease inhibitory activity of DIS aloes ranged from 84.6 to 94.4%, which was similar to or higher than 86.3% of fresh aloe. Also, urease inhibitory activity of DIS aloes was maintained at initial levels after heat treatment (90°C, 10 min.) and drying treatment (freeze or hot air drying). Urease inhibition pattern from Lineweaver-Burk plot indicated general non-competitive inhibition, and inhibition constants (K_IE and K_IES) of DIS aloes were 41-149 and 87-163 μL/mL, respectively. DIS(glucose) and DIS(polyethylene glycol) exhibited the highest retarding effect of glucose and bile acid. Their retarding effects were about 1.6 and 1.8 folds higher than that of fresh aloe after 0.5 and 1 hr of the dialysis, respectively. Conclusively, the above in vitro physiological functions of Aloe vera gel concentrated by DIS process suggested that aloe products treated with DIS would have the potential benefits for protection against Helicobacter pylori and reduction of blood glucose and cholesterol levels.

Keywords: dewatering impregnation & soaking; Aloe vera gel; urease inhibition; glucose/bile acid retarding effect