용매 추출과 병용 추출법을 이용한 양파 과육으로부터 quercetin 관련물질의 추출

진은영¹, 박영서², 장재권³, 정명수⁴, 박훈⁵, 심건섭⁶, 최영진¹^,^*

Extraction of Quercetin and Its Glucosides from Onion Edible Part Using Solvent Extraction and Various Extraction Assisting Methods

Eun Young Jin¹, Young-Seo Park², Jae Kweon Jang³, Myong-Soo Chung⁴, Hoon Park⁵, Kun-Sub Shim⁶, Young Jin Choi¹^,^*

¹서울대학교 농생명공학부

²경원대학교 식품생물공학과

³청강문화산업대학 식품과학과

⁴이화여자대학교 식품공학과

⁵선문대학교 식품과학전공

⁶(주)그린바이오

¹Department of Agricultural Biotechnology, Seoul National University

²Department of Food Science and Biotechnology, Kyungwon University

³Department of Food Science, Chungkang College of Cultural Industries

⁴Department of Food Science and Engineering, Ewha Womans University

⁵Department of Food Science, Sunmoon University

⁶Greenbio Co., Ltd.

^*Corresponding author: Young Jin Choi, Department of Agricultural Biotechnology, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-742, Korea, Tel: +82-2-880-4851; Fax: +82-2-873-5095, E-mail: choiyj@snu.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Feb 13, 2009; Revised: May 06, 2009; Accepted: May 22, 2009

Published Online: May 31, 2009

Abatract

Quercetin is one of the main flavonoids from onion. To use quercetin as a functional component for onion food products, the effects of various extraction assisting methods such as juicing methods, microwave, ultrasound and enzyme treatments on the yield of quercetin and its glucosides were investigated. For conventional solvent extraction, the highest yield of quercetin and its glycosides was achieved with 0.8 mL/g of 60% methanol at 50°C for 15 min. The juicing methods using mixer and screw showed no influence on the yield. Microwave and ultrasound treatments showed 2.14 times and 2.06 times more quercetin yields than non-treated extraction, respectively. For cellulase and viscozyme treatments, the highest yields of quercetin were achieved with 0.5 mL/g of 1% enzyme-0.1M sodium acetate (pH 5.2) buffer solution. Cellulase and viscozyme treatment improved quercetin yield 1.65 times and 2.29 times more than non-treated one, respectively.

Keywords: quercetin; extraction; onion; extraction assisting methods