흑목이 버섯 자실체의 열수추출물로부터 흑갈색 색소 성분의 흡착 특성

김현민¹, 허원¹, 임건빈², 이신영¹^,^*

Adsorption Characteristic of Brownish Dark Colored Compounds from the Hot Water Extract of Auricularia auricula Fruit Body

Hyeon-Min Kim¹, Won Hur¹, Kun Bin Lim², Shin-Young Lee¹^,^*

¹강원대학교 생물공학과

²(주)화인코

¹Department of Bioengineering and Technology, Kangwon National University

²Fineco Co., Ltd.

^*Corresponding author: Shin-Young Lee, Department of Bioengineering and Technology, Kangwon National University, 192-1 Hyoja-dong, Chunchon-si, Gangwon 200-701, Korea, Tel: +82-33-250-6273; Fax: +82-33-243-6350, E-mail: sylee@kangwon.ac.kr

ⓒ Copyright 2009 Korean Society for Food Engineering. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 13, 2009; Revised: May 12, 2009; Accepted: May 13, 2009

Published Online: May 31, 2009

Abatract

The crude polysaccharide fraction from fruit body of Auricularia auricula were obtained by using hot water extraction and ethanol precipitation. As the crude polysaccharide fraction contained the brownish dark colored compounds, the adsorption study of pigments from the crude polysaccharide using activated carbon was carried out. The pigment compounds showed an absorption characteristic with λ_max of 230 nm and the absorbance at 230 nm was taken as color intensity. Adsorption capacity of pigment depended on increase of the activated carbon to sample loading ratio. The adsorption capacity increased with increase of pH and temperature in the pH range of 3.0-7.0 and temperature range of 25-40°C, but decreased in the temperature range of 40-70°C. The optimum capacity was obtained at addition of 16.7 mg activated carbon per mL sample solution (concentration = 3 mg/mL) at pH of 7.0 and temperature of 40°C. Treatment for 10 min was sufficient to achieve the 80% decolorization and 1.25 fold purification of polysaccharide. Langmuir isotherm and pseudo second-order kinetic model provided the best fitting for adsorption of the brownish dark colored compounds onto powdered active carbon. The activation energies of adsorption from the Langmuir isotherm parameter in the ranges of 25-40°C and 40-70°C was -2.54 and 4.38 kcal/g, respectively. The results of low activation energy also indicated that the adsorption process was a physical adsorption which was controlled by diffnsion.

Keywords: Auricularia auricula; polysaccharide; pigment decolorization; activated carbon; adsorption